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Image Search Results
Journal: Frontiers in Endocrinology
Article Title: Exercise intervention improves mitochondrial quality in non-alcoholic fatty liver disease zebrafish
doi: 10.3389/fendo.2023.1162485
Figure Lengend Snippet: Swimming exercise protects against inflammation and fibrosis in diet-induced NAFLD zebrafish livers. (A) Western-blot of CLO1A1, ACTA2, IL-1β, and IL-10. Protein expression levels of (B) COL1A1, (C) ACTA2, (D) IL-1β, and (E) IL-10. (n=6) *, p < 0.05, **, p < 0.01, ***, p < 0.001. Data represent the mean, and error bars represent SEM. Scale bar, 20 μm. NAFLD, non-alcoholic fatty liver disease; N, normal diet; H, high fat diet; HE, high-fat diet plus exercise.
Article Snippet: The antibodies used were as follows: rabbit anti-GAPDH antibody (1:2000; servicebio), rabbit anti-COL1A1 antibody (1:1000; Wanleibio), rabbit anti-ACTA2 antibody (1:1000; Proteintech),
Techniques: Western Blot, Expressing
Journal: Journal of orthopaedic surgery and research
Article Title: M2 macrophages activate the IL-10/JAK2/STAT3 pathway to induce pathological microangiogenesis in the nucleus pulposus exacerbating intervertebral disc degeneration.
doi: 10.1186/s13018-025-05962-2
Figure Lengend Snippet: Fig. 6 Effect of silencing IL-10 on the JAK 2/STAT 3 pathway after co-culture of M2 macrophages with NP cells. A: WB analysis of JAK2 and STAT3 protein expression in NP cells; B: Quantitative analysis of JAK2 and STAT3 protein levels; C: RT-qPCR analysis of JAK2 and STAT3 mRNA expression. nsP>0.05; *P<0.05; **P<0.01; ***P<0.001
Article Snippet: Membranes were incubated overnight at 4 °C with primary antibodies specific to IL-10 (60269-1-Ig, Proteintech),
Techniques: Co-Culture Assay, Expressing, Quantitative RT-PCR
Journal: Journal of orthopaedic surgery and research
Article Title: M2 macrophages activate the IL-10/JAK2/STAT3 pathway to induce pathological microangiogenesis in the nucleus pulposus exacerbating intervertebral disc degeneration.
doi: 10.1186/s13018-025-05962-2
Figure Lengend Snippet: Fig. 7 Effect of the JAK 2/STAT 3 pathway on pathological microangiogenesis and ECM metabolism in degenerated NP. A: WB analysis of JAK2, STAT3, VEGF, VEGFR, and CD34 protein expression in normal NP cells, degenerated NP cells, and degenerated NP cells treated with the JAK2/STAT3 inhibitor AG490; B: Quantitative analysis of protein expression levels for JAK2, STAT3, VEGF, VEGFR, and CD34; C: RT-qPCR analysis of mRNA expression levels of COL2, aggrecan, MMP-3, and MMP-7. *P<0.05; **P<0.01; ***P<0.001
Article Snippet: Membranes were incubated overnight at 4 °C with primary antibodies specific to IL-10 (60269-1-Ig, Proteintech),
Techniques: Expressing, Quantitative RT-PCR